APLP1 Polyclonal antibody
APLP1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
12305-2-AP targets APLP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, IP, WB,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | APLP1 fusion protein Ag2951 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | amyloid beta (A4) precursor-like protein 1 |
| Synonyms | Amyloid like protein 1, APLP, APLP 1, APLP1 |
| Calculated Molecular Weight | 650 aa, 72 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC012889 |
| Gene Symbol | APLP1 |
| Gene ID (NCBI) | 333 |
| RRID | AB_2056369 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51693 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APLP1 antibody 12305-2-AP | Download protocol |
| IHC protocol for APLP1 antibody 12305-2-AP | Download protocol |
| IP protocol for APLP1 antibody 12305-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans. | ||
EMBO J Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons. | ||
Mol Cell Proteomics Label-free quantitative proteomics of mouse cerebrospinal fluid detects BACE1 protease substrates in vivo. | ||
J Proteome Res Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis. |