- Featured Product
- KD/KO Validated
Annexin A11 Polyclonal antibody
Annexin A11 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse uterus tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human kidney tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ANXA11 Ready-To-Use IHC Kit. ANXA11 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10479-2-AP targets Annexin A11 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Annexin A11 fusion protein Ag0729 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | annexin A11 |
| Synonyms | 56 kDa autoantigen, Annexin 11, annexin A11, Annexin XI, ANX11, ANXA11, CAP 50, CAP50 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56-60 kDa |
| GenBank Accession Number | BC007564 |
| Gene Symbol | ANXA11 |
| Gene ID (NCBI) | 311 |
| RRID | AB_2057171 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50995 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Annexin A11 antibody 10479-2-AP | Download protocol |
| IHC protocol for Annexin A11 antibody 10479-2-AP | Download protocol |
| IF protocol for Annexin A11 antibody 10479-2-AP | Download protocol |
| IP protocol for Annexin A11 antibody 10479-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis. | ||
Oncotarget ANXA11 regulates the tumorigenesis, lymph node metastasis and 5-fluorouracil sensitivity of murine hepatocarcinoma Hca-P cells by targeting c-Jun.
| ||
Biomed Pharmacother Annexin A11 knockdown inhibits in vitro proliferation and enhances survival of Hca-F cell via Akt2/FoxO1 pathway and MMP-9 expression.
| ||
Neurobiol Aging Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis. | ||
Ann Clin Transl Neurol Adult-onset dominant muscular dystrophy in Greek families caused by Annexin A11 |
