Name: 5 Lipoxygenase Mouse Monoclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, ELISA
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WB analysis of pig spleen using 66326-1-Ig

pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IHC staining of human lung using 66326-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Species specificity:

human, pig

Positive WB detected in:

pig spleen tissue

Positive IHC detected in:

human lung tissue, human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:1000-1:4000

IHC : 1:100-1:400

Product Information

Source:

Mouse

Purification method:

Protein A purification

Isotype:

IgG2a

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

5 Lipoxygenase fusion protein Ag18168

Full name:

arachidonate 5-lipoxygenase

Calculated molecular weight:

674 aa, 78 kDa

Observed molecular weight:

78 kDa

GenBank accession number:

BC130332

Gene ID (NCBI):

240

Gene symbol

ALOX5

Synonyms

5 lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5

Background

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

WB Figures

pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.