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5 Lipoxygenase Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66326-1-Ig |CloneNo.: 3B1B7

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浓度: 1000μg/ml

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Tested Applications

Applications:
WB, IHC, ELISA
Application not tested? Please see our Guarantee


Images See all

5 Lipoxygenase Antibody WB pig spleen tissue 66326-1-Ig

pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig( ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

5 Lipoxygenase Antibody IHC human lung tissue 66326-1-Ig

Immunohistochemistry of paraffin-embedded human lung tissue slide using 66326-1-Ig( ALOX5 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

5 Lipoxygenase Antibody IHC human lung tissue 66326-1-Ig

Immunohistochemistry of paraffin-embedded human lung tissue slide using 66326-1-Ig( ALOX5 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, pig
Positive WB detected in:
pig spleen tissue
Positive IHC detected in:
human lung tissue, human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:100-1:400

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
5 Lipoxygenase fusion protein Ag18168
Full name:
arachidonate 5-lipoxygenase
Calculated molecular weight:
674aa,78 kDa
Observed molecular weight:
78 kDa
GenBank accession number:
BC130332
Gene ID (NCBI):
240
Gene symbol
ALOX5
Synonyms
5 lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5

Background

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).


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