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AIF Monoclonal antibody
AIF Monoclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig, Canine and More (2)
Applications
IF/ICC,IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
8C12B2
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells, MDCK cells, Pig brain cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, human kidney tissue, HCT 116 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
67791-1-Ig targets AIF in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Canine samples.
Tested Applications | IF/ICC,IF-P, IHC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | Human, Mouse, Rat, Pig, Canine |
Cited Reactivity | human, rat |
Immunogen | AIF fusion protein Ag12400 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | apoptosis-inducing factor, mitochondrion-associated, 1 |
Synonyms | AIF, AIFM1, Cell and organelle markers, PDCD8 |
Calculated Molecular Weight | 609 aa, 66 kDa |
Observed Molecular Weight | 67 kDa, 57 kDa |
GenBank Accession Number | BC111065 |
Gene Symbol | AIFM1 |
Gene ID (NCBI) | 9131 |
RRID | AB_2918555 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O95831 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for AIF antibody 67791-1-Ig | Download protocol |
IHC protocol for AIF antibody 67791-1-Ig | Download protocol |
IF protocol for AIF antibody 67791-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma. | ||
Inflammation Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro | ||
Adv Sci (Weinh) Use of Deep-Learning Assisted Assessment of Cardiac Parameters in Zebrafish to Discover Cyanidin Chloride as a Novel Keap1 Inhibitor Against Doxorubicin-Induced Cardiotoxicity |