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AFP Monoclonal antibody

AFP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B3E4

Cat No : 67852-1-Ig

Print datasheet

Synonyms

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

验证数据展示

  • WB analysis of HepG2 using 67852-1-Ig

    WB analysis of HepG2 using 67852-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HuH-7 using 67852-1-Ig

    WB analysis of HuH-7 using 67852-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 67852-1-Ig

    WB analysis of human testis using 67852-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 67852-1-Ig

    WB analysis of human placenta using 67852-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67852-1-Ig

    IHC staining of human liver cancer using 67852-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human liver using 67852-1-Ig

    IHC staining of human liver using 67852-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AFP antibody included

经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, human testis tissue, human placenta tissue
Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67852-1-Ig targets AFP in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen AFP fusion protein Ag6089 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alpha-fetoprotein
Synonyms AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC027881
Gene Symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).

实验方案

Product Specific Protocols
WB protocol for AFP antibody 67852-1-IgDownload protocol
IHC protocol for AFP antibody 67852-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols