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ADH7 Polyclonal antibody

ADH7 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 23425-1-AP

Print datasheet

Synonyms

ADH 4, ADH7, Gastric alcohol dehydrogenase, Retinol dehydrogenase

验证数据展示

  • WB analysis of HeLa using 23425-1-AP

    WB analysis of HeLa using 23425-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 23425-1-AP

    IHC staining of human liver using 23425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

  • IHC staining of human liver using 23425-1-AP

    IHC staining of human liver using 23425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 23425-1-AP

    IF Staining of HepG2 using 23425-1-AP

    Immunofluorescent analysis of HepG2 cells, using ADH7 antibody 23425-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 23425-1-AP is ADH7 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen ADH7 fusion protein Ag19340 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
Synonyms ADH 4, ADH7, Gastric alcohol dehydrogenase, Retinol dehydrogenase
Calculated Molecular Weight 386 aa, 41 kDa
Observed Molecular Weight 45-47 kDa
GenBank Accession NumberBC131512
Gene Symbol ADH7
Gene ID (NCBI) 131
RRIDAB_2879277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40394
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH7(Alcohol dehydrogenase class 4 mu/sigma chain) is also named as gastric alcohol dehydrogenase, retinol dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. The ubiquitous expression pattern of ADH7 during embryogenesis has led to the notion that the enzymatic conversion of retinol to retinal occurs more or less uniformly and plays no role in the spatial or temporal regulation of RA synthesis during embryonic development(PMID:17473173). It has 2 isoforms produced by alternative splicing and can exsit as a homodimer(PMID:18479436).

实验方案

Product Specific Protocols
WB protocol for ADH7 antibody 23425-1-APDownload protocol
IHC protocol for ADH7 antibody 23425-1-APDownload protocol
IF protocol for ADH7 antibody 23425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

Mettl3-mediated m6A modification plays a role in lipid metabolism disorders and progressive liver damage in mice by regulating lipid metabolism-related gene expression

Authors - Guanqi Dai
View Article