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Alpha Adducin Polyclonal antibody

Alpha Adducin Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10791-1-AP

Print datasheet

Synonyms

ADD1, ADDA, adducin 1 (alpha), Alpha adducin

验证数据展示

  • WB analysis of K-562 using 10791-1-AP

    WB analysis of K-562 using 10791-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10791-1-AP (Alpha adducin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 10791-1-AP

    IHC staining of human ovary tumor using 10791-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10791-1-AP (Alpha adducin antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of mouse brain using 10791-1-AP

    IF Staining of mouse brain using 10791-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha Adducin antibody (10791-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, mouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10791-1-AP targets Alpha Adducin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat, bovine
Immunogen Alpha Adducin fusion protein Ag1245 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adducin 1 (alpha)
Synonyms ADD1, ADDA, adducin 1 (alpha), Alpha adducin
Calculated Molecular Weight 81 kDa, 84 kDa, 70 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC013393
Gene Symbol ADD1
Gene ID (NCBI) 118
RRIDAB_2257952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35611
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Adducin antibody 10791-1-APDownload protocol
IHC protocol for Alpha Adducin antibody 10791-1-APDownload protocol
IF protocol for Alpha Adducin antibody 10791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Decoding the Synaptic Proteome with Long-Term Exposure to Midazolam during Early Development.

Authors - Nghi M Nguyen
View Article
bovineWB

Mol Biol Rep

Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cells

Authors - Yue Wang
View Article