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ADA Polyclonal antibody

ADA Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13328-1-AP

Print datasheet

Synonyms

ADA, Adenosine aminohydrolase, adenosine deaminase

验证数据展示

  • WB analysis of HeLa using 13328-1-AP

    WB analysis of HeLa using 13328-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13328-1-AP

    IHC staining of human stomach using 13328-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human stomach using 13328-1-AP

    IHC staining of human stomach using 13328-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of mouse small intestine using 13328-1-AP

    IF Staining of mouse small intestine using 13328-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse small intestine using 13328-1-AP

    IF Staining of mouse small intestine using 13328-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, Sp2/0 cells
Positive IHC detected inhuman stomach tissue, human cervical cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse small intestine tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 13328-1-AP is ADA Fusion Protein expressed in E. coli.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ADA fusion protein Ag4128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenosine deaminase
Synonyms ADA, Adenosine aminohydrolase, adenosine deaminase
Calculated Molecular Weight 363 aa, 41 kDa
Observed Molecular Weight 45-47 kDa
GenBank Accession NumberBC040226
Gene Symbol ADA
Gene ID (NCBI) 100
RRIDAB_2877939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADA antibody 13328-1-APDownload protocol
IHC protocol for ADA antibody 13328-1-APDownload protocol
IF protocol for ADA antibody 13328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

Front Cell Dev Biol

Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.

Authors - Xiaoting Chen
View Article
mouseWB

Eur J Pharmacol

Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.

Authors - Gerald Wölkart
View Article