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SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP) tested by ELISA. SARS-CoV-2 Nucleocapsid Phosphoprotein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.
The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity.(PMID: 32416961, PMID: 32235387)