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14-3-3 Monoclonal antibody 0 Publications

Mouse Monoclonal| Catalog number: 66061-1-Ig |CloneNo.: 2E10A3

浓度: 710 μg/ml

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


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WB analysis using 66061-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IHC staining of human lung cancer using 66061-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of human lung cancer using 66061-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IF Staining of HepG2 using 66061-1-Ig

Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

FC experiment of HeLa using 66061-1-Ig

1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (66061-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Species specificity:
human, rat, mouse, pig
Positive WB detected in:
HEK-293 cells
Positive IHC detected in:
human lung cancer tissue, rat brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:20000
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
14-3-3 fusion protein Ag6055
Full name:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Calculated molecular weight:
28 kDa
Observed molecular weight:
31 kDa, 28 kDa
GenBank accession number:
BC056867
Gene ID (NCBI):
10971
Gene symbol
YWHAQ
Synonyms
14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3 tau, HS1, Protein HS1, YWHAQ

Background

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.


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