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Recombinant Human IL-12 protein (His Tag)

ED50

0.04-0.16 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

3593(IL-12B) & 3592(IL-12A)

Accession

P29460(IL-12B) & P29459(IL-12A)

Cat No : Eg0575

Print datasheet

Synonyms

IL-12, IL-12 p40/IL-12 p35, IL-12 p70, IL-12B/IL-12A, Interleukin-12

验证数据展示

  • Purity of Recombinant Human IL-12 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-12 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Measured by its ability to induce IFN-gamma secretion in human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.04-0.16 ng/mL.

    Measured by its ability to induce IFN-gamma secretion in human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.04-0.16 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Measured by its ability to induce IFN-gamma secretion in human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.04-0.16 ng/mL.
Source HEK293-derived Human IL-12 protein Ile23-Ser328 (Human IL-12B Accession# P29460)+linker+Arg23-Ser219 (Human IL-12A Accession# P29459) with His tag at the C-terminus.
Predicted Molecular Mass 59.5 kDa
SDS-PAGE 65-80 kDa
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • 12 months from the date of receipt, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 (referred to as 'p70') is a 70 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40). IL-12 is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. IL-12 has been shown to play a critical role in the pathogenesis of a variety of immune-related diseases.

References:

1. Picard C. et al. (2002). Am J Hum Genet.70: 336-48.
2. Randolph AG. et al. (2004). Am J Hum Genet. 75: 709-15.
3. Hsieh CS. et al. (1993). Science.260: 547-9.
4. Stern AS. et al.(1996). Life Sci. 58: 639-54.
5. Colombo MP. et al. (2002). Cytokine Growth Factor Rev. 13: 155-68.