- Featured Product
- KD/KO Validated
smooth muscle actin specific Recombinant antibody
smooth muscle actin specific Recombinant Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat, chicken, pig, zebrafish and More (1)
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5H7
验证数据展示
经过测试的应用
Positive WB detected in | mouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine tissue, rat colon tissue |
Positive IHC detected in | human liver tissue, human heart tissue, human appendicitis tissue, mouse colon tissue, rat colon tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse heart tissue, mouse embryo tissue |
Positive FC detected in | C2C12 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80008-1-RR targets smooth muscle actin specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, chicken, pig, zebrafish samples.
Tested Applications | FC, IF-P, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, mouse, rat, chicken, pig, zebrafish |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | actin, alpha 2, smooth muscle, aorta |
Synonyms | a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, SMA, smooth muscle actin, smooth muscle actin specific, smooth muscle actin-specific |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | NM_001613 |
Gene Symbol | ACTA2 |
Gene ID (NCBI) | 59 |
RRID | AB_2882938 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P62736 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for smooth muscle actin specific antibody 80008-1-RR | Download protocol |
IHC protocol for smooth muscle actin specific antibody 80008-1-RR | Download protocol |
IF protocol for smooth muscle actin specific antibody 80008-1-RR | Download protocol |
FC protocol for smooth muscle actin specific antibody 80008-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
Pharmacol Res MicroRNA-24-3p alleviates cardiac fibrosis by suppressing cardiac fibroblasts mitophagy via downregulating PHB2. | ||
Int Immunopharmacol TSLP/TSLPR promotes renal fibrosis by activating STAT3 in renal fibroblasts | ||
Eur J Pharmacol Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway | ||
Drug Des Devel Ther Ginsenoside Rb1 Alleviates Bleomycin-Induced Pulmonary Inflammation and Fibrosis by Suppressing Central Nucleotide-Binding Oligomerization-, Leucine-Rich Repeat-, and Pyrin Domains-Containing Protein Three Inflammasome Activation and the NF-κB Pathway. | ||
Exp Biol Med (Maywood) Targeting endothelial coagulation signaling ameliorates liver obstructive cholestasis and dysfunctional angiogenesis |