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EIF3E Monoclonal antibody

EIF3E Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C8

Cat No : 67095-1-Ig

Print datasheet

Synonyms

eIF 3 p48, eIF3 p46, EIF3 P48, EIF3E, EIF3S6, INT6

验证数据展示

  • IP experiment of HeLa using 67095-1-Ig

    IP experiment of HeLa using 67095-1-Ig

    IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug.

  • WB analysis using 67095-1-Ig

    WB analysis using 67095-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67095-1-Ig targets EIF3E in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen EIF3E fusion protein Ag28523 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit E
Synonyms eIF 3 p48, eIF3 p46, EIF3 P48, EIF3E, EIF3S6, INT6
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberBC000734
Gene Symbol EIF3E
Gene ID (NCBI) 3646
RRIDAB_2882400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60228
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF3E antibody 67095-1-IgDownload protocol
IP protocol for EIF3E antibody 67095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols