NF-L Polyclonal antibody
NF-L Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC,IF-P, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | SH-SY5Y cells, Neuro-2a cells, mouse brain tissue, C6 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells, mouse brain tissue |
Positive FC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12998-1-AP targets NF-L in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC,IF-P, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | NF-L fusion protein Ag3667 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | neurofilament, light polypeptide |
Synonyms | 68 kDa neurofilament protein, CMT1F, CMT2E, NEFL, NF, NF L, NF68, NFL, NF-L |
Calculated Molecular Weight | 543 aa, 62 kDa |
Observed Molecular Weight | 65-68 kDa, 140-160 kDa |
GenBank Accession Number | BC039237 |
Gene Symbol | NEFL |
Gene ID (NCBI) | 4747 |
RRID | AB_10597388 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P07196 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NF-L antibody 12998-1-AP | Download protocol |
IHC protocol for NF-L antibody 12998-1-AP | Download protocol |
IF protocol for NF-L antibody 12998-1-AP | Download protocol |
IP protocol for NF-L antibody 12998-1-AP | Download protocol |
FC protocol for NF-L antibody 12998-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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Mol Cell Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury. | ||
Mater Today Bio Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment. | ||
Mater Today Bio In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation. | ||
Adv Sci (Weinh) An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress. | ||
Front Mol Neurosci Achaete-Scute Homolog 1 Expression Controls Cellular Differentiation of Neuroblastoma. | ||
Hum Mol Genet Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons. |