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- KD/KO Validated
IRF3 Polyclonal antibody
IRF3 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
136
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HCT 116 cells, HeLa cells, U-937 cells, human brain tissue, Jurkat cells, MCF-7 cells, MDA-MB-231 cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11312-1-AP targets IRF3 in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, RIP, IHC, CoIP, ChIP |
Tested Reactivity | human |
Cited Reactivity | human, rat, sheep, monkey, pig |
Immunogen | IRF3 fusion protein Ag1858 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | interferon regulatory factor 3 |
Synonyms | interferon regulatory factor 3, IRF 3, IRF3 |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 50-60 kDa |
GenBank Accession Number | BC009395 |
Gene Symbol | IRF3 |
Gene ID (NCBI) | 3661 |
RRID | AB_2127004 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14653 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IRF3 antibody 11312-1-AP | Download protocol |
IHC protocol for IRF3 antibody 11312-1-AP | Download protocol |
IF protocol for IRF3 antibody 11312-1-AP | Download protocol |
IP protocol for IRF3 antibody 11312-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
Acta Pharm Sin B Allosteric inhibition reveals SHP2-mediated tumor immunosuppression in colon cancer by single-cell transcriptomics. | ||
Emerg Microbes Infect NSUN2-mediated M5c methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infections |