Recombinant human IL-4 (HZ-1004) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.4 ng/mL.