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PR Polyclonal antibody

PR Polyclonal Antibody for IF/ICC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC,ELISA

Conjugate

Unconjugated

Cat No : 19980-1-AP

Print datasheet

Synonyms

NR3C3, PGR, PR, progesterone receptor

验证数据展示

  • IHC staining of human breast cancer using 19980-1-AP

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PR antibody included
  • IF Staining of MCF-7 using 19980-1-AP

    IF Staining of MCF-7 using 19980-1-AP

    Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PR Ready-To-Use IHC Kit. PR primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19980-1-AP targets PR in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC,ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name progesterone receptor
Synonyms NR3C3, PGR, PR, progesterone receptor
Calculated Molecular Weight 933 aa, 99 kDa
GenBank Accession NumberNM_000926
Gene Symbol PGR
Gene ID (NCBI) 5241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06401
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.