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PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

FC (Intra), IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 13371-1-AP

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Synonyms

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL



经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, C6 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells, THP-1 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human liver cancer tissue, mouse testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNeuro-2a cells, mouse testis tissue, MCF-7 cells
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13371-1-AP targets PARP1 in WB, IP, IHC, IF, FC (Intra), CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF, IHC, IP, WB, ELISA
Cited Applications ChIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, monkey, pig, canine, bovine, Fungus
Immunogen PARP1 fusion protein Ag4193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly (ADP-ribose) polymerase 1
Synonyms ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight 113-116 kDa, 89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
RRIDAB_2160459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

实验方案

Product Specific Protocols
WB protocol for PARP1 antibody 13371-1-APDownload protocol
IHC protocol for PARP1 antibody 13371-1-APDownload protocol
IF protocol for PARP1 antibody 13371-1-APDownload protocol
IP protocol for PARP1 antibody 13371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
humanWB

Mol Cancer

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

Authors - Jiangfeng Li
humanWB

ACS Nano

PMID: 31670945

Authors - Ying Xu
mouseWB

Exp Mol Med

Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury

Authors - Zhenjun Yu
humanWB

Nat Commun

The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation.

Authors - Guangjian Fan
humanWB

Nat Commun

Loss of KLF14 triggers centrosome amplification and tumorigenesis.

Authors - Guangjian Fan