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- KD/KO Validated
Occludin Polyclonal antibody
Occludin Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
378
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse liver tissue, human kidney tissue, human liver tissue, mouse colon tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:800 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13409-1-AP targets Occludin in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, pig, canine, bovine |
Immunogen | Occludin fusion protein Ag4057 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | occludin |
Synonyms | occludin, OCLN |
Calculated Molecular Weight | 522 aa, 59 kDa |
Observed Molecular Weight | 59 kDa |
GenBank Accession Number | BC029886 |
Gene Symbol | OCLN |
Gene ID (NCBI) | 4950 |
RRID | AB_2156308 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16625 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Occludin antibody 13409-1-AP | Download protocol |
IHC protocol for Occludin antibody 13409-1-AP | Download protocol |
IF protocol for Occludin antibody 13409-1-AP | Download protocol |
IP protocol for Occludin antibody 13409-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
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