- Featured Product
- KD/KO Validated
MARCH8 Polyclonal antibody
MARCH8 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14119-1-AP targets MARCH8 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Immunogen | MARCH8 fusion protein Ag5268 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | membrane-associated ring finger (C3HC4) 8 |
| Synonyms | c MIR, MARCH VIII, MARCH8, MIR, RING finger protein 178, RNF178 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC066988 |
| Gene Symbol | MARCH8 |
| Gene ID (NCBI) | 220972 |
| RRID | AB_2140168 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T0T0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MARCH8 (Membrane-associated RING finger protein 8) is also named as MIR, RNF178 and belongs to the RING zinc finger protein family. It functions as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1). It plays a key role in controlling MHC class II surface expression by regulated ubiquitination of a lysine residue in the beta-chain (PMID:22761441).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MARCH8 antibody 14119-1-AP | Download protocol |
| IP protocol for MARCH8 antibody 14119-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes. | ||
Autophagy BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.
| ||
Autophagy 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8.
| ||
Proc Natl Acad Sci U S A The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation. | ||
Cancers (Basel) MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells. |