c-Kit/CD117 Polyclonal antibody
c-Kit/CD117 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Mo7e cells |
Positive IHC detected in | stromal tumor tissue, human placenta tissue, human testis tissue, Insulinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18696-1-AP targets c-Kit/CD117 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, sheep |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
Synonyms | C Kit, CD117, c-Kit, c-Kit/CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit |
Calculated Molecular Weight | 110 kDa |
Observed Molecular Weight | 140-160 kDa |
GenBank Accession Number | BC071593 |
Gene Symbol | KIT |
Gene ID (NCBI) | 3815 |
RRID | AB_2249558 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P10721 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for c-Kit/CD117 antibody 18696-1-AP | Download protocol |
IHC protocol for c-Kit/CD117 antibody 18696-1-AP | Download protocol |
IF protocol for c-Kit/CD117 antibody 18696-1-AP | Download protocol |
FC protocol for c-Kit/CD117 antibody 18696-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mech Ageing Dev Acceleration of melanocyte senescence by the proinflammatory cytokines IFNγ and TNFα impairs the repigmentation response of vitiligo patients to narrowband ultraviolet B (NBUVB) phototherapy | ||
Front Cell Dev Biol Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis. | ||
Front Immunol Immune Infiltrating Cells-Derived Risk Signature Based on Large-scale Analysis Defines Immune Landscape and Predicts Immunotherapy Responses in Glioma Tumor Microenvironment. | ||
J Exp Clin Cancer Res Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells. | ||
Andrology Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes | ||
Tumour Biol Orai1 mediates tumor-promoting store-operated Ca(2+) entry in human gastrointestinal stromal tumors via c-KIT and the extracellular signal-regulated kinase pathway. |