Cardiac Troponin I Monoclonal antibody
Cardiac Troponin I Monoclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF/ICC,IF-P, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1D5D6
验证数据展示
经过测试的应用
Positive WB detected in | human heart tissue, rat heart tissue, mouse heart tissue, pig heart tissue, rabbit heart tissue |
Positive IHC detected in | human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse heart tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IF | See 6 publications below |
产品信息
66376-1-Ig targets Cardiac Troponin I in WB, IF, IHC, ISFET, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | IF/ICC,IF-P, IHC, WB, ELISA |
Cited Applications | WB, IF, ISFET |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | Cardiac Troponin I fusion protein Ag16758 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | troponin I type 3 (cardiac) |
Synonyms | Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle |
Calculated Molecular Weight | 210 aa, 24 kDa |
Observed Molecular Weight | 26-28 kDa |
GenBank Accession Number | BC096165 |
Gene Symbol | TNNI3 |
Gene ID (NCBI) | 7137 |
RRID | AB_2881753 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P19429 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Cardiac Troponin I antibody 66376-1-Ig | Download protocol |
IHC protocol for Cardiac Troponin I antibody 66376-1-Ig | Download protocol |
IF protocol for Cardiac Troponin I antibody 66376-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biosens Bioelectron A handheld testing device for the fast and ultrasensitive recognition of cardiac troponin I via an ion-sensitive field-effect transistor | ||
Stem Cell Reports Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue | ||
Stem Cell Res Ther Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection. | ||
J Cell Mol Med Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress. | ||
Front Pharmacol Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway. |