Claudin 3 Polyclonal antibody

Claudin 3 Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IHC, IP,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16456-1-AP

Print datasheet

Synonyms

C7orf1, claudin 3, Claudin3, CLDN3, CPE R 2, CPE R2, CPE receptor 2, CPETR2, HRVP1, RVP1



经过测试的应用

Positive IP detected inmouse large intestine tissue
Positive IHC detected inhuman breast cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

产品信息

16456-1-AP targets Claudin 3 in IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP,ELISA
Cited Applications IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, pig
Immunogen Claudin 3 fusion protein Ag9411 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name claudin 3
Synonyms C7orf1, claudin 3, Claudin3, CLDN3, CPE R 2, CPE R2, CPE receptor 2, CPETR2, HRVP1, RVP1
Calculated molecular weight 220 aa, 23 kDa
Observed molecular weight 20-24 kDa
GenBank accession numberBC016056
Gene symbol CLDN3
UniprotO15551
Gene ID (NCBI) 1365
RRIDAB_10733226
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

实验方案

Product Specific Protocols
IHC protocol for Claudin 3 antibody 16456-1-APDownload protocol
IP protocol for Claudin 3 antibody 16456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Aging Cell

Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts.

Authors - Zhi Chen
mouseIF

EMBO Mol Med

Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.

Authors - Ming Zhao
mouse,ratIHC

J Hazard Mater

Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.

Authors - Junrong Wu
mouseIHC

Environ Int

Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments.

Authors - Jie Cai
mouse

Biochem Pharmacol

Inhibition of S100A9 alleviates neurogenic pulmonary edema after subarachnoid hemorrhage

Authors - Guijun Wang
mouseIHC

NanoImpact

Altered protein S-glutathionylation depicts redox imbalance triggered by transition metal oxide nanoparticles in a breastfeeding system.

Authors - Jie Cai