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- KD/KO Validated
Claudin 1 Polyclonal antibody
Claudin 1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine and More (5)
Applications
IF, IHC, WB, ELISA
Conjugate
Unconjugated
213
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse skin tissue, mouse thymus tissue, mouse liver tissue, rat skin tissue, A549 cells, HUVEC cells |
Positive IHC detected in | human cervical cancer tissue, human skin cancer tissue, mouse skin tissue, human oesophagus cancer tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HUVEC cells, MDCK cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
产品信息
13050-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
Tested Applications | IF, IHC, WB, ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | human, mouse, rat, canine |
Cited Reactivity | human, mouse, rat, goat, chicken, sheep, pig, canine, bovine |
Immunogen | Claudin 1 fusion protein Ag3713 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | claudin 1 |
Synonyms | claudin 1, CLD1, CLDN1, ILVASC, SEMP1 |
Calculated molecular weight | 211 aa, 23 kDa |
Observed molecular weight | 20-23 kDa |
GenBank accession number | BC012471 |
Gene symbol | CLDN1 |
Uniprot | O95832 |
Gene ID (NCBI) | 9076 |
RRID | AB_2079881 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Claudin 1 antibody 13050-1-AP | Download protocol |
IHC protocol for Claudin 1 antibody 13050-1-AP | Download protocol |
IF protocol for Claudin 1 antibody 13050-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation | ||
Clin Cancer Res ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism. | ||
EMBO Rep O-glycan initiation directs distinct biological pathways and controls epithelial differentiation. | ||
Mater Today Bio Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis | ||
PLoS Pathog Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2. | ||
Phytomedicine Baizhu shaoyao decoction restores the intestinal barrier and brain-gut axis balance to alleviate diarrhea-predominant irritable bowel syndrome via FoxO1/FoxO3a |