ELISA assay for ACE2 (18-740) activity SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 ug/mL and then incubated with a dilution series of ACE2 (18-740) Antibody. Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP), followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.
