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  • Featured Product
  • KD/KO Validated

TUBGCP3 Polyclonal antibody

TUBGCP3 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15719-1-AP

Print datasheet

Synonyms

GCP 3, GCP3, h104p, hGCP3, hGrip104, hSpc98, SPBC98, Spc98p, TUBGCP3

验证数据展示

  • WB analysis of HeLa using 15719-1-AP

    WB analysis of HeLa using 15719-1-AP

    WB result of TUBGCP3 antibody (15719-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUBGCP3 transfected HeLa cells.

  • WB analysis of Jurkat using 15719-1-AP

    WB analysis of Jurkat using 15719-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15719-1-AP (TUBGCP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15719-1-AP targets TUBGCP3 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB,ELISA
Cited Applications IF, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen TUBGCP3 fusion protein Ag8437 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, gamma complex associated protein 3
Synonyms GCP 3, GCP3, h104p, hGCP3, hGrip104, hSpc98, SPBC98, Spc98p, TUBGCP3
Calculated Molecular Weight 103.5 kDa
Observed Molecular Weight 95-101 kDa
GenBank Accession NumberBC007763
Gene Symbol TUBGCP3
Gene ID (NCBI) 10426
RRIDAB_2211272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUBGCP3, also named as γ‐tubulin complex protein 3, localized to the centrosome, is a member of γ-tubulin complex proteins which play key roles in microtubule nucleation and organization. It has been reported that TUBGCP3 rotation can bring γ-tubulin molecules into proximity. 15719-1-AP antibody detects the isoform proteins around 95-101 kDa and 50 kDa in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for TUBGCP3 antibody 15719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

The dynamics of microtubule minus ends in the human mitotic spindle.

Authors - Nicolas Lecland
View Article
humanWB

Nat Commun

Modular assembly of the principal microtubule nucleator γ-TuRC.

Authors - Martin Würtz
View Article
mouseWB

Nat Commun

Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity.

Authors - Carlos Sánchez-Huertas
View Article
humanWB,IF

J Cell Sci

MZT1 regulates microtubule nucleation by linking γTuRC assembly to adapter-mediated targeting and activation.

Authors - Rosa Ramírez Cota
  • KD Validated
View Article
humanWB

J Cell Sci

Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.

Authors - Arunas Kvainickas
View Article
humanWB

J Neuropathol Exp Neurol

Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma.

Authors - Eduarda Dráberová
View Article