AbFlex 5-methylcytosine antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Not Species Specific

Applications

DB

Cat No : 91187,91188 91187

Synonyms

5-methylcytidine, methyl cytidine, 5 MeCyd, 5-Me, 5 MeC, MeDIP, methylated DNA immunoprecipitation, DNA methylation, monoclonal, mouse, mAb, antibody, antibodies, methylcytosine, stem cell, stem cells



产品信息

Tested Applications DB

Applications Validated by Active Motif: Dot Blot: 1 ug/ml

Tested Reactivity Not Species Specific
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
ModificationMethylated
Immunogen This antibody was raised against 5-methylcytosine conjugated to KLH.
Full Name AbFlex 5-methylcytosine antibody (rAb)
Synonyms 5-methylcytidine, methyl cytidine, 5 MeCyd, 5-Me, 5 MeC, MeDIP, methylated DNA immunoprecipitation, DNA methylation, monoclonal, mouse, mAb, antibody, antibodies, methylcytosine, stem cell, stem cells
Molecular weight
GenBank accession numberN/A
RRIDAB_2793794
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex 5-methylcytosine (5-mC) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. This antibody is useful for discrimination between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.