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  • KD/KO Validated

ARP2 Polyclonal antibody

ARP2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10922-1-AP

Print datasheet

Synonyms

Actin like protein 2, Actin related protein 2, ACTR2, ARP2



经过测试的应用

Positive WB detected inHeLa cells, mouse kidney tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ACTR2 Ready-To-Use IHC Kit. ACTR2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10922-1-AP targets ARP2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen ARP2 fusion protein Ag1359 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ARP2 actin-related protein 2 homolog (yeast)
Synonyms Actin like protein 2, Actin related protein 2, ACTR2, ARP2
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC014546
Gene Symbol ACTR2
Gene ID (NCBI) 10097
RRIDAB_2221854
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61160
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARP2 antibody 10922-1-APDownload protocol
IHC protocol for ARP2 antibody 10922-1-APDownload protocol
IF protocol for ARP2 antibody 10922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
  • KD Validated
humanWB

Nucleic Acids Res

G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.

Authors - Xiaomei He
WB

Elife

Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein

Authors - Congyan Zhang
humanWB

Elife

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.

Authors - Dawei Wang
  • KD Validated
humanIF

Elife

mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex.

Authors - Madeline G Andrews
mouseWB

Biol Reprod

RAB8A GTPase regulates spindle migration and Golgi apparatus distribution via ROCK-mediated actin assembly in mouse oocyte meiosis.

Authors - Zhen-Nan Pan