- Featured Product
- KD/KO Validated
Beta Actin Polyclonal antibody
Beta Actin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey, canine and More (7)
Applications
FC, IF, IHC, WB, ELISA and More (3)
Conjugate
Unconjugated
3241
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse colon tissue, A549 cells, C6 cells, HeLa cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, rat kidney tissue, Caco-2 cells, RAW 264.7 cells, SMMC-7721 cells, Jurkat cells, rat spleen tissue, HepG2 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MDCK cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20536-1-AP targets Beta Actin in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, monkey, canine samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | Cell treatment, CoIP, ELISA, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat, monkey, canine |
Cited Reactivity | human, mouse, rat, goat, chicken, carp, yeast, sheep, Ochotona curzoniae, Camelus bactrianus |
Immunogen | Beta Actin fusion protein Ag14521 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | actin, beta |
Synonyms | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin |
Calculated Molecular Weight | 375 aa, 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC002409 |
Gene Symbol | ACTB |
Gene ID (NCBI) | 60 |
RRID | AB_10700003 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P60709 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Beta Actin antibody 20536-1-AP | Download protocol |
IHC protocol for Beta Actin antibody 20536-1-AP | Download protocol |
IF protocol for Beta Actin antibody 20536-1-AP | Download protocol |
FC protocol for Beta Actin antibody 20536-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. | ||
Cell Microglia jointly degrade fibrillar alpha-synuclein cargo by distribution through tunneling nanotubes. | ||
Cell Discov Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability | ||
Adv Mater Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages | ||
Cell Mol Immunol cFLIPS regulates alternative NLRP3 inflammasome activation in human monocytes |