Various lysates were subjected to SDS PAGE followed by western blot with 10144-2-AP (STAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10144-2-AP (STAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Result of anti-STAT1 (IP:10144-2-AP, 4ug; Detection:10144-2-AP 1:500) with PC-3 cells lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 10144-2-AP (STAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB result of STAT1 antibody (10144-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells.
(blue)1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human STAT1 (10144-2-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).