Purity of recombinant human IL-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human IL-9 (HZ-1240) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of recombinant IL-9 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.15-0.85 ng/mL​.

Purity of recombinant human IL-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-9 (HZ-1240-GMP) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of GMP recombinant IL-9 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.15-0.85 ng/mL​.