1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse Bcl2 (CL647-66799, Clone:1B5B9) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).