MAP2 Monoclonal antibody

MAP2 Monoclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

FC, IF, IHC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1C3E6

Cat No : 67015-1-Ig

Print datasheet

Synonyms

MAP 2, MAP2, MAP2A, MAP2B, MAP2C



经过测试的应用

Positive IHC detected inmouse brain tissue, rat cerebellum tissue, rat brain tissue, human brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, mouse brain tissue
Positive FC detected inSH-SY5Y cells, Neuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67015-1-Ig targets MAP2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC, IF, IHC, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MAP2 fusion protein Ag11349 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name microtubule-associated protein 2
Synonyms MAP 2, MAP2, MAP2A, MAP2B, MAP2C
Calculated Molecular Weight 200 kDa
GenBank Accession NumberBC038857
Gene Symbol MAP2
Gene ID (NCBI) 4133
RRIDAB_2882331
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

实验方案

Product Specific Protocols
IHC protocol for MAP2 antibody 67015-1-IgDownload protocol
IF protocol for MAP2 antibody 67015-1-IgDownload protocol
FC protocol for MAP2 antibody 67015-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

Revisiting astrocyte to neuron conversion with lineage tracing in vivo.

Authors - Lei-Lei Wang
mouseIF

Cell Stem Cell

In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.

Authors - Wenjiao Tai
  • KO Validated
ratIF

Small

Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect.

Authors - Xiheng Lu
ratIF

Cell Death Dis

ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Authors - Yaxuan Zhang
mouseWB,IF

Cell Death Dis

Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes

Authors - Yuan Yao
mouseIF

Acta Neuropathol Commun

TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.

Authors - Tuancheng Feng