PD-1/CD279 Monoclonal antibody
PD-1/CD279 Monoclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat, mouse, pig
Applications
FC, IF/ICC,IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
4H4D1
验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue, human lymphoma tissue |
| Positive FC detected in | PMA and ionomycin treated MOLT-4 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66220-1-Ig targets PD-1/CD279 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
| Tested Applications | FC, IF/ICC,IF-P, IHC, WB, ELISA |
| Cited Applications | FC, IF, IHC, WB |
| Tested Reactivity | human, rat, mouse, pig |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PD-1/CD279 fusion protein Ag12470 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | programmed cell death 1 |
| Synonyms | CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2 |
| Calculated Molecular Weight | 288 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa, 47-55 kDa |
| GenBank Accession Number | BC074740 |
| Gene Symbol | PDCD1 |
| Gene ID (NCBI) | 5133 |
| RRID | AB_2881611 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| IHC protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| IF protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy | ||
Nat Commun m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. | ||
Cancers (Basel) Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma. | ||
Biochem Pharmacol Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT | ||
Aging (Albany NY) Elevated lymphocyte specific protein 1 expression is involved in the regulation of leukocyte migration and immunosuppressive microenvironment in glioblastoma. | ||
CNS Neurosci Ther The immunomodulatory effect of microglia on ECM neuroinflammation via the PD-1/PD-L1 pathway. |
