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- KD/KO Validated
FIS1 Polyclonal antibody
FIS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (6)
Applications
IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
263
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse brain tissue, rat brain tissue, mouse heart tissue, SH-SY5Y cells, pig brain tissue, HeLa cells, rat heart tissue, Jurkat cells, mouse spleen tissue, rat spleen tissue, HepG2 cells, PC-12 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | rat brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hepa1-6 cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:14000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10956-1-AP targets FIS1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, goat, chicken, zebrafish, hamster, pig, squirrel, bovine |
Immunogen | FIS1 fusion protein Ag1409 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) |
Synonyms | CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11 |
Calculated Molecular Weight | 17 kDa |
Observed Molecular Weight | 17 kDa |
GenBank Accession Number | BC009428 |
Gene Symbol | FIS1 |
Gene ID (NCBI) | 51024 |
RRID | AB_2102532 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y3D6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for FIS1 antibody 10956-1-AP | Download protocol |
IHC protocol for FIS1 antibody 10956-1-AP | Download protocol |
IF protocol for FIS1 antibody 10956-1-AP | Download protocol |
IP protocol for FIS1 antibody 10956-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature Distinct fission signatures predict mitochondrial degradation or biogenesis.
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Nat Immunol RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.
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Cell Stem Cell AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells.
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Nat Metab Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice |