PEX19 Polyclonal antibody

PEX19 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14713-1-AP

Print datasheet

Synonyms

33 kDa housekeeping protein, D1S2223E, HK33, Peroxin 19, PEX19, PMP1, PMPI, PXF, PXMP1



经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, mouse heart tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14713-1-AP targets PEX19 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, Arabidopsis
Immunogen PEX19 fusion protein Ag6434 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peroxisomal biogenesis factor 19
Synonyms 33 kDa housekeeping protein, D1S2223E, HK33, Peroxin 19, PEX19, PMP1, PMPI, PXF, PXMP1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC000496
Gene Symbol PEX19
Gene ID (NCBI) 5824
RRIDAB_2162265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).

实验方案

Product Specific Protocols
WB protocol for PEX19 antibody 14713-1-APDownload protocol
IHC protocol for PEX19 antibody 14713-1-APDownload protocol
IP protocol for PEX19 antibody 14713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling.

Authors - Celia de la Calle Arregui
humanWB

Cell Death Differ

Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.

Authors - Weiwei Cui
humanWB

J Cell Biol

Ubiquitin ligase MARCH5 localizes to peroxisomes to regulate pexophagy.

Authors - Jun Zheng
humanWB

Front Mol Biosci

PUP-IT2 as an alternative strategy for PUP-IT proximity labeling

Authors - Suyu Yue
ArabidopsisWB

PLoS One

The Early-Acting Peroxin PEX19 Is Redundantly Encoded, Farnesylated, and Essential for Viability in Arabidopsis thaliana.

Authors - Margaret M McDonnell
humanWB,IF

Curr Biol

Evolutionary analysis of cellular reduction and anaerobicity in the hyper-prevalent gut microbe Blastocystis

Authors - Kristína Záhonová