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Catalase Polyclonal antibody

Catalase Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21260-1-AP

Print datasheet

Synonyms

CAT, catalase, Cell and organelle markers, Peroxisome Marker



经过测试的应用

Positive WB detected inHeLa cells, rat liver tissue, Raji cells, Jurkat cells, HepG2 cells, L02 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21260-1-AP targets Catalase in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications FC, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Meretrix meretrix, goat, sheep, pig, bat, bovine, Fungus
Immunogen Catalase fusion protein Ag15383 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name catalase
Synonyms CAT, catalase, Cell and organelle markers, Peroxisome Marker
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC112219
Gene Symbol CAT
Gene ID (NCBI) 847
RRIDAB_10733099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04040
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.

实验方案

Product Specific Protocols
WB protocol for Catalase antibody 21260-1-APDownload protocol
IHC protocol for Catalase antibody 21260-1-APDownload protocol
IF protocol for Catalase antibody 21260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Authors - Calvin S Carter
mouseWB

Nat Commun

Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity

Authors - Jinjie Duan
mouseWB

Nat Commun

Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery.

Authors - Jun Zhou
mouseWB

Nat Commun

The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.

Authors - Guang Yang
humanWB

Redox Biol

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Authors - Wen-Su Wei
mouseWB

Cell Rep

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Authors - Wu Hongtan H