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  • KD/KO Validated

ALKBH7 Polyclonal antibody

ALKBH7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15470-1-AP

Print datasheet

Synonyms

ABH7, ALKBH7, SPATA11, UNQ6002



经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15470-1-AP targets ALKBH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ALKBH7 fusion protein Ag7772 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alkB, alkylation repair homolog 7 (E. coli)
Synonyms ABH7, ALKBH7, SPATA11, UNQ6002
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC004393
Gene Symbol ALKBH7
Gene ID (NCBI) 84266
RRIDAB_2226855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH7(Alkylated DNA repair protein alkB homolog 7) is also named as ABH7, SPATA11 and belongs to the alkB family. This protein may be a dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Quantitative real-time PCR detectes variable ALKBH7 expression in all 16 tissues examined, with highest expression in pancreas, followed by spleen, prostate, ovary, and placenta(PMID:17979886). The gene encodes a 25 kDa protein with a 20 amino acid signal peptide.

实验方案

Product Specific Protocols
WB protocol for ALKBH7 antibody 15470-1-APDownload protocol
IHC protocol for ALKBH7 antibody 15470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.

Authors - Li-Sheng Zhang
  • KD Validated
human,mouseWB

Cell Death Dis

ALKBH7 drives a tissue and sex-specific necrotic cell death response following alkylation-induced damage.

Authors - Jennifer J Jordan
  • KO Validated