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  • Featured Product
  • KD/KO Validated

ALKBH3 Polyclonal antibody

ALKBH3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12292-1-AP

Print datasheet

Synonyms

ABH3, ALKBH3, DEPC 1, DEPC1, PCA1, Prostate cancer antigen 1

验证数据展示

  • WB analysis using 12292-1-AP

    WB analysis using 12292-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12292-1-AP

    IHC staining of human lung cancer using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human prostate cancer using 12292-1-AP

    IHC staining of human prostate cancer using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human lung cancer using 12292-1-AP

    IHC staining of human lung cancer using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human pancreas cancer using 12292-1-AP

    IHC staining of human pancreas cancer using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of rat stomach using 12292-1-AP

    IHC staining of rat stomach using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of mouse stomach using 12292-1-AP

    IHC staining of mouse stomach using 12292-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALKBH3 antibody included

经过测试的应用

Positive WB detected inJurkat cells, mouse kidney tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman lung cancer tissue, human testis tissue, human prostate cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALKBH3 Ready-To-Use IHC Kit. ALKBH3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12292-1-AP targets ALKBH3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ALKBH3 fusion protein Ag2982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alkB, alkylation repair homolog 3 (E. coli)
Synonyms ABH3, ALKBH3, DEPC 1, DEPC1, PCA1, Prostate cancer antigen 1
Calculated Molecular Weight 286 aa, 33 kDa
Observed Molecular Weight 22-25 kDa, 33kDa
GenBank Accession NumberBC015155
Gene Symbol ALKBH3
Gene ID (NCBI) 221120
RRIDAB_11125161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96Q83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

实验方案

Product Specific Protocols
WB protocol for ALKBH3 antibody 12292-1-APDownload protocol
IHC protocol for ALKBH3 antibody 12292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Gastroenterol Hepatol

ALKBH3 expression is associated with tumor recurrence and unfavorable prognosis in hepatocellular carcinoma.

Authors - Qian Wang
  • KD Validated
View Article