Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IP result of anti-SIX2 (IP:11562-1-AP, 4ug; Detection:11562-1-AP 1:1000) with HEK-293 cells lysate 1320 ug.

IF result from Fogelgren B, PMID:18570229, "Misexpression of Six2 is associated with heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice.".

IF result of SIX2 antibody (11562-1-AP, 1:200) with mouse embryonic kidney rudiment dissected at E13.5 and cultured for 2 days by Dr. Aleksandra Rak-Raszewska. SIX2 positive cells (red) incondense metanephric mesenchyme surrounding the ureteric bud tip.

Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SIX2 (CL594-66347, Clone:1F9G11) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.