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SLC3A1 Polyclonal antibody

SLC3A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16343-1-AP

Print datasheet

Synonyms

ATR1, CSNU1, D2H, NBAT, RBAT, SLC3A1



经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16343-1-AP targets SLC3A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat
Immunogen SLC3A1 fusion protein Ag9525 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
Synonyms ATR1, CSNU1, D2H, NBAT, RBAT, SLC3A1
Calculated Molecular Weight 685 aa, 79 kDa
Observed Molecular Weight79 kDa
GenBank Accession NumberBC022386
Gene Symbol SLC3A1
Gene ID (NCBI) 6519
RRIDAB_2239419
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)

实验方案

Product Specific Protocols
WB protocol for SLC3A1 antibody 16343-1-APDownload protocol
IHC protocol for SLC3A1 antibody 16343-1-APDownload protocol
IP protocol for SLC3A1 antibody 16343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney.

Authors - M Takasato
mouseWB

Nat Commun

Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidney.

Authors - Joseph O Lamontagne
mouseIF

Dev Cell

AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.

Authors - Alexander G Marneros
humanWB

Theranostics

Cysteine transporter SLC3A1 promotes breast cancer tumorigenesis.

Authors - Yang Jiang
  • KD Validated
mouseIF

Sci Rep

Kidney-specific transposon-mediated gene transfer in vivo.

Authors - Lauren E Woodard
mouseWB

FASEB J

Epidermal growth factor deficiency predisposes to progressive renal disease.

Authors - Alia M Zeid