Various lysates were subjected to SDS PAGE followed by western blot with 14865-1-AP (SDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP result of anti-SDHA(IP:14865-1-AP, 4ug; Detection:14865-1-AP 1:20000) with HEK-293 cells lysate 960 ug.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP (SDHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHA antibody (14865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).