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  • Featured Product
  • KD/KO Validated

PTP1B Polyclonal antibody

PTP1B Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11334-1-AP

Print datasheet

Synonyms

PTP 1B, PTP1B, PTPN1

验证数据展示

  • WB analysis of Sp2/0 using 11334-1-AP

    WB analysis of Sp2/0 using 11334-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 11334-1-AP

    WB analysis of Jurkat using 11334-1-AP

    WB result of PTP1B antibody (11334-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP1B transfected Jurkat cells.

  • IP experiment of A431 using 11334-1-AP

    IP experiment of A431 using 11334-1-AP

    IP result of anti-PTP1B (IP:11334-1-AP, 4ug; Detection:11334-1-AP 1:500) with A431 cells lysate 2400ug.

  • IF Staining of BxPC-3 using 11334-1-AP

    IF Staining of BxPC-3 using 11334-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 11334-1-AP (PTP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human prostate cancer using 11334-1-AP

    IHC staining of human prostate cancer using 11334-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11334-1-AP (PTP1B antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inSp2/0 cells, BxPC-3 cells, A431 cells, HeLa cells, Jurkat cells, THP-1 cells, mouse liver tissue, human testis tissue, HL-60 cells, human placenta tissue, C6 cells, NIH/3T3 cells, mouse placenta tissue, rat liver tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11334-1-AP targets PTP1B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PTP1B fusion protein Ag1878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 1
Synonyms PTP 1B, PTP1B, PTPN1
Calculated Molecular Weight 435 aa, 50 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC015660
Gene Symbol PTPN1
Gene ID (NCBI) 5770
RRIDAB_10642566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

实验方案

Product Specific Protocols
WB protocol for PTP1B antibody 11334-1-APDownload protocol
IHC protocol for PTP1B antibody 11334-1-APDownload protocol
IF protocol for PTP1B antibody 11334-1-APDownload protocol
IP protocol for PTP1B antibody 11334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.

Authors - Changying Shi
View Article
ratWB

J Cell Physiol

Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p.

Authors - Qing-Ping Yao
View Article
mouseWB,IHC

FASEB J

PMID: 32100381

Authors - Ping Yang
View Article
humanWB

Front Cell Dev Biol

Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.

Authors - Juan Du
  • KD Validated
View Article
mouseWB

Eur J Pharmacol

Oleanolic acid derivative alleviates cardiac fibrosis through inhibiting PTP1B activity and regulating AMPK/TGF-β/Smads pathway

Authors - An-Hui Wang
View Article
mouseWB

iScience

Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis

Authors - Shohei Takaoka
View Article