IP result of anti-KGA/GAC (IP:12855-1-AP, 4ug; Detection:12855-1-AP 1:5000) with mouse brain tissue lysate 2400 ug.
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.
Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KGA/GAC (12855-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
