WB result of EIF2A/CDA02 antibody (11233-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP Result of anti-EIF2A (IP:11233-1-AP, 3ug; Detection:11233-1-AP 1:1000) with MCF-7 cells lysate 3250ug.
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 40x lens).
Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
1X10^6 HepG2 cells were stained with .2ug EIF2A antibody (11233-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2A/CDA02 antibody (11233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).