HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-DYNC1H1(IP:12345-1-AP, 4ug; Detection:12345-1-AP 1:1500) with HeLa cells lysate 1320 ug.
