Various lysates were subjected to SDS PAGE followed by western blot with 12780-1-AP (CP110 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12780-1-AP (CP110 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-CP110 (IP:12780-1-AP, 4ug; Detection:12780-1-AP 1:1500) with Jurkat cells lysate 2400 ug.

WB result of CP110 antibody (12780-1-AP, 1:500) with si-control and si-CP110 transfected HeLa cells.

Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CP110 antibody (12780-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CP110 (12780-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 66448-1-Ig (CP110 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®488 CP110 antibody (CL488-12780) at dilution of 1:100.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 CP110 antibody (CL594-12780) at dilution of 1:100.

Various lysates were subjected to SDS PAGE followed by western blot with 66448-1-Ig (CP110 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66448-1-PBS in a different storage buffer formulation.

CP110 fusion protein Ag3489 SDS-PAGE