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  • KD/KO Validated

WT1 Polyclonal antibody

WT1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12609-1-AP

Print datasheet

Synonyms

GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT 2, WT1, WT33



经过测试的应用

Positive WB detected inK-562 cells, human liver tissue, MCF-7 cells, A431 cells, rat kidney tissue, mouse kidney tissue
Positive IP detected inK-562 cells
Positive IF detected inK-562 cells
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12609-1-AP targets WT1 in WB, IP, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IP, WB, ELISA
Cited Applications chIP, IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken
Immunogen WT1 fusion protein Ag3337 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Wilms tumor 1
Synonyms GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT 2, WT1, WT33
Calculated Molecular Weight 449 aa, 49 kDa, 57 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC032861
Gene Symbol WT1
Gene ID (NCBI) 7490
RRIDAB_2216225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.

实验方案

Product Specific Protocols
WB protocol for WT1 antibody 12609-1-APDownload protocol
IF protocol for WT1 antibody 12609-1-APDownload protocol
IP protocol for WT1 antibody 12609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.

Authors - Michael Heider
mouseWB,IF

Mol Psychiatry

Brain-specific Wt1 deletion leads to depressive-like behaviors in mice via the recruitment of Tet2 to modulate Epo expression.

Authors - Fen Ji
  • KO Validated
mousechIP

EMBO Mol Med

Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis.

Authors - Rajesh K Kasam
humanWB

Oncogene

YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.

Authors - G Hua
mouseWB

Oncogene

USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.

Authors - Juntae Kwon
humanIF

Elife

Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.

Authors - Jianlin Liang