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WNT1 Polyclonal antibody

WNT1 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

FC, IF, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27935-1-AP

Print datasheet

Synonyms

INT1, Proto oncogene Int 1 homolog, Proto oncogene Wnt 1, WNT1



经过测试的应用

Positive WB detected in3T3-L1 cells, HepG2 cells, NIH/3T3 cells, Jurkat cells
Positive IF detected inNIH/3T3 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27935-1-AP targets WNT1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, IF, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen WNT1 fusion protein Ag27380 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name wingless-type MMTV integration site family, member 1
Synonyms INT1, Proto oncogene Int 1 homolog, Proto oncogene Wnt 1, WNT1
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC074799
Gene Symbol WNT1
Gene ID (NCBI) 7471
RRIDAB_2881013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04628
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WNT1 antibody 27935-1-APDownload protocol
IF protocol for WNT1 antibody 27935-1-APDownload protocol
FC protocol for WNT1 antibody 27935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bioengineered

MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1)

Authors - Guang-Lin Song
humanWB

Neural Regen Res

Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke

Authors - Jianfeng Wang
humanWB

Aging (Albany NY)

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Authors - Liansheng Sha
mouseWB

Microb Biotechnol

Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice.

Authors - Xiaoxiao Zhao
humanWB,IHC

Front Oncol

Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.

Authors - Hanqing Wang
mouseWB

Transplantation

Agmatine Attenuates Liver Ischemia Reperfusion Injury by Activating Wnt/β-catenin Signaling in Mice.

Authors - Zhenyi Han