HE4 Polyclonal antibody

HE4 Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14406-1-AP

Print datasheet

Synonyms

HE4, WAP5, WFDC2



经过测试的应用

Positive IHC detected inhuman ovary tumor tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy HE4 Ready-To-Use IHC Kit. HE4 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14406-1-AP targets HE4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC,ELISA
Cited Applications IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen HE4 fusion protein Ag5684 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WAP four-disulfide core domain 2
Synonyms HE4, WAP5, WFDC2
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC046106
Gene Symbol WFDC2
Gene ID (NCBI) 10406
RRIDAB_2878055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14508
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

实验方案

Product Specific Protocols
IHC protocol for HE4 antibody 14406-1-APDownload protocol
IF protocol for HE4 antibody 14406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Biosens Bioelectron

An ultrasensitive ratiometric electrochemiluminescence immunosensor combining photothermal amplification for ovarian cancer marker detection.

Authors - Dandan Fang
mouseWB

Oncotarget

Bcl-3 is a novel biomarker of renal fibrosis in chronic kidney disease.

Authors - Ran Chen
mouseWB

Food Funct

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Authors - Jian Fang
human

RSC Adv

Machine learning algorithms enhance the specificity of cancer biomarker detection using SERS-based immunoassays in microfluidic chips

Authors - Nariman Banaei
humanIHC

Iran J Pathol

HE4, A New Potential Tumor Marker for Early Diagnosis and Predicting of Breast Cancer Progression.

Authors - Nazanin Mirmohseni Namini
humanIHC

Cell Rep

YAP targetome reveals activation of SPEM in gastric pre-neoplastic progression and regeneration

Authors - Adrian K H Loe